Establishment and Evaluation of a Loop-Mediated Isothermal Amplification Assay for Detection of Raccoon Dog in Meat Mixtures

Table 1

LAMP primers used in this study.

Primer name	Nucleotide sequence ()
Set 1-F3	GTTACGTGTTACCCTGAGG
Set 1-B3	AATGAATGGGAGGATGAAGT
Set 1-FIP	CAATGTATGGGATGGCGGAAAG-ACAAATGTCATTCTGAGGAGC
Set 1-BIP	TTGGTTGAGTGAATTTGAGGAGG-GGAATGCGAAAAATCGTGT
Set 2-F3	TAACCTCCTTTCCGCCAT
Set 2-B3	GAGGGATTATTGGATCCGG
Set 2-FIP	AGGTTGCCTTGTCTACTGAGAA-ATACATTGGAACTAATTTGGTTGAG
Set 2-BIP	ACACGATTTTTCGCATTCCACT-TTCGTGGAGGAACAGAAGA
Set 3-F3	CGAGGCCTATACTATGGATC
Set 3-B3	CTCCTCAAATTCACTCAACC
Set 3-FIP	CCTATGAATGCCGTAGCTATGGTC-TTCATAGAAACATGGAACATCG
Set 3-BIP	TGTTACCCTGAGGACAAATGTCA-ATTAGTTCCAATGTATGGGATG
Set 4-F3	TATTGATCTCCCCGCACC
Set 4-B3	GCGTGTATATAGCGGATGA
Set 4-FIP	AGTGTATGGCTAGAAATAGACCTGT-ATCTCTGCCTGATGAAACT
Set 4-BIP	ACATCAGATACCTCTACTGCCTT-GCCGTAATTGACGTCTCG
TaqMan forward primer	CTATACCTGGCATCTGGTTCTTACC
TaqMan reverse primer	GTCCCATTTGAGTGGATTAGTAGGA
TaqMan probe	FAM-CAGGGCCATGAACTCCCTCCATCC-TAMRA