Transcriptomic Profiling of Human Placental Trophoblasts in Response to Infection with Enterococcus faecalis

Table 3

Primers used for real-time PCR gene expression assays.

Target gene	Sequence (5′-3′)
IL1	ACCTTCAAGGAGAGCATGGTG
	GGTGCTGACCTAGGCTTGATG
IL4	GCTATGACTAAAGCAGGGACAA
	CACTCTCCTCAGCAAACACAGA
IL6	TGCTCCTGGTGTTGCCTG
	GCCGTCGAGGATGTACCG
IL8	GGCTCTCTTGGCAGCCTTC
	GCAGTTGGTCCACTCTCAATC
IL10	CCAGGCAACCTGCCTAACA
	TCTCAGACAAGGCTTGGCAAC
IL12	GTCCTCCTGGACCACCTCAG
	TCAATCTCTTCAGAAGTGCAAGG
TNF-α	CACCACGCTCTTCTGCCTG
	GTTCAGCCACTGGAGCTGC
IFN-γ	GCAGGTCATTCAGATGTAGCG
	TTCCTTGATGGTCTCCACACTC
TLR1	CAGTGTCTGGTACACGCATGGT
	TTTCAAAAACCGTGTCTGTTAGAGA
TLR2	CACTCAGGAGCAGCAAGCAC
	GCAGGAACAGAGCACAGCAC
TLR3	CCTGGTTTGTTAATTGGATTAACGA
	TGAGGTGGAGTGTTGCAAAGG
TLR4	CAGAGTTTCCTGCAATGGATCA
	GCTTATCTGAAGGTGTTGCACAT
TLR6	GAAGAAGAACAACCCTTTAGGATAGC
	AGGCCAAACAAAATGGAAGCTT
Claudin 1	TGCTGGAATTGAACTACTCCAC
	TACTCTTTCCGGTTGGTGTGA
E-cadherin	GAATGATGATGGTGGACAAT
	TACCACATTCGTCACTGCTA
Occludin	GGCACCTGCATACTCACCC
	CTGGGAGAGCAACTCATCCTC
ZO-1	CAACATACAGTGACGCTTCACA
	CACTATTGACGTTTCCCCACTC
ICAM	TTGTCCTGCCAGCGACTC
	AAGGAGTCGTGCCATAGGTG
madCAM	GCAGGAGGAGGAGGAGGAGGAG
	TCCAAGCCAGGCAGCC
β-2-microglobulin; (housekeeping gene)	GGCTATCCAGCGTACTCCAAA
	CGGCAGGCATACTCATCTTTTT