Preparation and Characterization of Antioxidant Peptides from Carrot Seed Protein

Table 2

The Box–Behnken design for optimizing hydrolysis conditions with DPPH radical-scavenging activity.

Run number	Coded levels of variable			Experimental values			Response value Y (%)
				Hydrolysis time (h)	Substrate concentration (%)	Protease dosage (U/g)
1	0	1	1	2.0	6.0	500	77.58
2	−1	0	−1	0.5	3.5	100	60.55
3	−1	1	0	0.5	6.0	300	73.08
4	0	0	0	2.0	3.5	300	73.52
5	−1	0	1	0.5	3.5	500	73.61
6	1	1	0	3.5	6.0	300	78.04
7	0	−1	1	2.0	1.0	500	35.13
8	0	−1	−1	2.0	1.0	100	33.98
9	1	0	1	3.5	3.5	500	70.64
10	0	0	0	2.0	3.5	300	70.26
11	0	0	0	2.0	3.5	300	73.96
12	1	0	−1	3.5	3.5	100	64.34
13	0	0	0	2.0	3.5	300	73.43
14	−1	−1	0	0.5	1.0	300	36.36
15	0	0	0	2.0	3.5	300	66.37
16	1	−1	0	3.5	1.0	300	38.55
17	0	1	−1	2.0	6.0	100	63.72