Immobilization of Bifidobacterium infantis Cells in Selected Hydrogels as a Method of Increasing Their Survival in Fermented Milkless Beverages

Table 6

Survival of free B. infantis ATCC15697 KKP2029p cells immobilized in calcium alginate and pectinate in fermented beverages during storage at 4°C.

B. infantis ATCC15697	Initial cell count (log cfu·g^−1)	Cell count reduction (log)
		4 days	7 days	14 days	21 days	28 days
Oat-banana beverage
Free	9.28 ± 0.27	3.75 ± 0.24^d	5.12 ± 0.13^d	9.28 ± 0.27^h	—	—
Microcapsules^(1) with 1.5% alginate	9.21 ± 0.26	2.24 ± 0.25^c	3.67 ± 0.23^b	6.20 ± 0.24^a	9.21 ± 0.26	—
Microbeads with 1.5% alginate	9.71 ± 0.20	3.24 ± 0.29^a	4.82 ± 0.06^g	7.02 ± 0.11^g	9.71 ± 0.20	—
Microcapsules^(2) with 2% alginate	8.75 ± 0.18	1.47 ± 0.20^e	2.53 ± 0.19^e	5.10 ± 0.23^f	>7	8.75 ± 0.18
Microbeads with 2% alginate	9.14 ± 0.35	2.05 ± 0.38^c	3.16 ± 0.24^f	5.91 ± 0.46^a	>8	9.14 ± 0.35
Oat beverage
Free	9.41 ± 0.10	4.06 ± 0.17^d	5.40 ± 0.37^d	9.41 ± 0.10^h	—	—
Beads with 5% pectin	9.77 ± 0.15	0.93 ± 0.08^b	2.11 ± 0.07^a	3.32 ± 0.29^b	5.08 ± 0.24^a	>7
Beads with 2% alginate	9.62 ± 0.04	1.09 ± 0.07^b	2.19 ± 0.12^a	3.76 ± 0.10^c	5.60 ± 0.16^b	>8
Oat-peach beverage
Free	9.41 ± 0.10	5.15 ± 0.32^f	6.88 ± 0.29^h	9.41 ± 0.10^h	—	-
Beads with 5% pectin	9.77 ± 0.15	2.88 ± 0.15^a	3.93 ± 0.24^bc	4.28 ± 0.26^d	5.96 ± 0.10^c	>8
Beads with 2% alginate	9.62 ± 0.04	2.92 ± 0.10^a	4.08 ± 0.19^c	4.70 ± 0.11^e	6.51 ± 0.16^d	9.62 ± 0.04

The values are the mean of two replicates. The values with the same superscript letter in columns do not differ significantly (). ⁽¹⁾Microcapsules with liquid core. ⁽²⁾Microcapsules with alginate core.