Monitoring the Levels of Biogenic Amines in Canned Fish Products Marketed in Ghana

Table 1

HPLC gradient condition for the separation of biogenic amines.

Time (min)	Mobile phase composition
	Eluent A (%)	Eluent B (%)
0.1	85	15
13.0	50	50
20.0	27	73
22.6	27	73
22.7	5	95
29.0	5	95
30.0	85	15
35.0	85	15

Eluent A = 0.1 M sodium acetate and 10 mM sodium octanesulfonate (pH 4.5). Eluent B = eluent A and acetonitrile (6.6 : 3.4).