Monitoring the Levels of Biogenic Amines in Canned Fish Products Marketed in Ghana

Table 2

Performance of the ion-pair HPLC method for biogenic amine determination in canned tuna.

Analyte	Regression equation	R^2	LOD	LOQ	Repeatability (%)		Recovery (%)
			(mg/kg)	(mg/kg)	Intraday	Interday	a	b	c
CAD	Y = 668196x − 566250	0.9997	0.42	1.41	5.6	8.5	92.4	96.8	95.4
PUT	Y = 931609x − 5580	0.9998	0.47	1.58	7.3	6.5	95.9	101.4	97.2
HIS	Y = 414513x − 212445	0.9998	0.39	1.29	4.4	4.1	93.2	97.4	94.7
TYR	Y = 217003x + 56548	0.9991	0.78	2.53	2.8	1.2	95.7	96.3	92.5
AGM	Y = 166697x − 67346	0.9994	0.60	2.00	9.2	6.1	88.9	95.6	90.7

R², square of regression coefficient; LOD, limit of detection; LOQ, limit of quantification; RSD, relative standard deviation; TYR, tyramine; PUT, putrescine; CAD, cadaverine; HIS, histamine; AGM, agmatine; a, 10 mg/kg; b, 50 mg/kg; c, 100 mg/kg.