Selection, Identification, and Application of Aptamers against Agaricus bisporus Lectin to Establish an Aptamer-AuNPs Colorimetric Method for Detection of ABL

Table 2

The sequences and the features of the selected representative candidate aptamers.

Sequence name	Sequence 5′-3′	Length	Free energy (kcal/mol)	Copy
seq-1	ATAGGAGTCACGACGACCAGGCATTCCGTTGATCCCTGAGAATAGAAGTAAGTTCCTTGGTATGTGCGTCTACCTCTGA	79	−4.8	9
seq-2	ATTAGGAGTCACGACGACCAGAACGATCATGTGCGACGGATCCAATTCTATATATGTCATTTATGTGCGTCTACCTCTTGA	81	−4.5	2
seq-3	ATAGGAGTCACGACGACCAGCCGAAATAGCCCCCGCTTGACTGGTATAGGTTTGGAGTTGTATGTGCGTCTACCTCTG	79	−4.4	2
seq-4	ATAGGAGTCACGACGACCAGCATATATTCACAATGAGGCTCTTAGTCTGAGATATCCTCCTATGTGCGTCTACCTCTTGA	80	−6.3	2
seq-17	ATAGGAGTCACGACGACCAGCTGACCTTCCTGTGTACGATGCTTCGGCTACAGCTGATGCTATGTGCGTCTACCTCTTGA	80	−10.4	1
seq-41	ATAGGAGTCACGACGACCAGTCCTGGGTGACTTGCACCAGGTGCGGCCTCGTAGCTTTTCTATGTGCGTCTACCTCTTGA	80	−11.6	1