Research Article

Application of Nucleic Acid Reference Material for Rapid Detection of Cronobacter sakazakii (Cronobacter spp.) in Flammulina velutipes

Figure 3

Sensitivity analysis of primers for 16S–23S. M, DNA marker D (100–2000 bp); lanes 1-2, 40 ng (100); lanes 3-4, 4 ng (10−1); lanes 5-6, 0.4 ng (10−2); lanes 7-8, 0.04 ng (10−3); lanes 9-10, 0.004 ng (10−4); lanes 11-12, 0.0004 ng (10−5); lanes 13-14, 0.00004 ng (10−6); and lane 15, blank control.