Estimation of Total Quercetin and Rutin Content in Malus domestica of Nepalese Origin by HPLC Method and Determination of Their Antioxidative Activity

Table 4

Regression data, LODs, and LOQs for quercetin and rutin analyzed by HPLC.

Standards	Regression equation	R^2	LOD (µg/mL)	LOQ(µg/mL)
Quercetin	y = 34751x + 40572	0.9999	0.379	1.148
Rutin	y = 9766.4x + 19679	0.9996	0.641	1.946

Note. All the tests were done in triplicate; y = peak area; x: concentration (µg/mL).