Estimation of Total Quercetin and Rutin Content in Malus domestica of Nepalese Origin by HPLC Method and Determination of Their Antioxidative Activity

Table 5

Analytical results of intraday and interday variability.

Standard	Concentration (μg/mL)	Intraday		Interday
		Mean ± SD	RSD (%)	Mean ± SD	RSD (%)
Quercetin	250	251.42 ± 1.15	0.46	248.81 ± 0.34	0.14
	125	125.43 ± 0.41	0.33	124.64 ± 1.21	0.97
	62.5	62.14 ± 0.29	0.47	63.20 ± 0.54	0.85
Rutin	250	252.51 ± 0.41	0.16	252.69 ± 1.25	0.49
	125	126.21 ± 0.89	0.71	125.05 ± 0.63	0.5
	62.5	62.06 ± 0.31	0.49	61.22 ± 0.44	0.72

Note. All the tests were done in triplicate.