Estimation of Total Quercetin and Rutin Content in Malus domestica of Nepalese Origin by HPLC Method and Determination of Their Antioxidative Activity

Table 7

Recovery data of spiked standard to the M. domestica extract.

Standard	Original (μg/mL)	Spiked (μg/mL)	Measured (μg/mL)	Recovery (%)	RSD (%)
Quercetin	21.11	250	270.96 ± 0.09	99.29	0.03
		125	145.53 ± 0.22	97.27	0.16
		62.5	82.91 ± 0.02	96.67	0.03
Rutin	47.8	250	299.18 ± 0.29	102.89	0.10
		125	172.66 ± 0.92	99.70	0.53
		62.5	108.71 ± 0.34	96.67	0.31

Note. All the tests were done in triplicate.