A New Reference Plasmid “pGMT27” Provides an Efficient Transgenic Detection Method for Flue-Cured Tobacco

Figure 3

Calibration curves for P-35S and NR in qPCR. The dilutions contained approximately 10⁶, 10⁵, 10⁴, 10³, and 10² copies per PCR. (a) Amplification plot of P-35S. (b) Amplification plot of NR. (c) Standard curve of P-35S (y = −3.288x + 40.022, R² = 0.997, Eff% = 101.427). (d) Standard curve of NR (y = −3.37x + 39.38, R² = 0.998, Eff% = 98.036).

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