A New Reference Plasmid “pGMT27” Provides an Efficient Transgenic Detection Method for Flue-Cured Tobacco

Table 1

Description of the fragments present in the pGMT27 plasmid (18,296 bp).

Targets		Description of genetic elements	NCBI (accession no. and position of corresponding bp on the sequence of the accession)	Size (bp)
Exogenous genes	P-35S^A	Cauliflower mosaic virus 35S promoter	AF485783 (4974..5808)	835
	NPT II^A	Neomycin phosphotransferase II	AF485783 (2838..3632)	795
	T-NOS^A	Agrobacterium tumefaciens nopaline synthase terminator	AF485783 (4022..4277)	256
	GUS^A	Beta-glucuronidase (uidA) gene	AF485783 (5845..7656)	1,812
	HPT^B	Hygromycin phosphotransferase gene	AF354045 (8948..9973)	1,026
	Bar^B	Herbicide-resistant gene	KF840400 (2381..2932)	552
	aadA^B	Spectinomycin-resistant gene	EU497669 (3544..4335)	803
Endogenous genes	Actin^B	N. tabacum gene for actin	X63603 (4269..4640)	372
	NR^B	N. tabacum gene for nitrate reductase (nia-1)	JN384019 (445..1455)	1,011

A: from plant expression vector pBI121; B: synthesis.