A New Reference Plasmid “pGMT27” Provides an Efficient Transgenic Detection Method for Flue-Cured Tobacco

Table 3

The LOD and LOQ of the pGMT27 plasmid as the calibrator in qPCR.

Target	Template copies	Positive signals/total repeats	Mean Ct	Ct SD
P-35S	50	9/9	34	0.33
	25	9/9	35.08	0.54
	10	9/9	36.63	0.89
	5	8/9	—	NA
NR	50	9/9	34.04	0.61
	25	9/9	35.22	0.38
	10	9/9	36.03	0.76
	5	8/9	—	NA

NA: not applicable.