A New Reference Plasmid “pGMT27” Provides an Efficient Transgenic Detection Method for Flue-Cured Tobacco

Table 4

Conversion factor (Cf) estimation of the reference molecule pGMT27 by qPCR.

Quality of genomic DNA (ng)	Target copies		Cf	Mean Cf value	SD	RSD (%)
	P-35S	NR
6.25	1675.12	4905.71	0.34	0.39	0.03	6.77
12.5	3850.91	9848.56	0.39
25	7782.50	19525.56	0.40
50	15455.08	38570.14	0.40
100	30893.15	76172.85	0.41