A New Reference Plasmid “pGMT27” Provides an Efficient Transgenic Detection Method for Flue-Cured Tobacco

Table 5

Unknown sample analyses for GMO content (%) using the pGMT27 plasmid as the calibrator (Cf = 0.39).

True value (%)	Experimental value (%)			Accuracy		Precision
	1	2	3	Mean (%)	Bias (%)	SD	RSD (%)
0.5	0.40	0.39	0.47	0.42	−15.55	0.04	9.84
2	1.71	1.75	1.75	1.73	−13.29	0.02	1.34
5	4.26	4.28	3.90	4.15	−17.08	0.21	5.16
6.25	6.25	5.16	5.32	5.57	−10.80	0.59	10.60
12.5	12.65	12.54	11.91	12.37	−1.07	0.40	3.26
25	26.18	25.28	24.99	25.48	1.93	0.62	2.42

Bias = [(mean experimental value − true value)/true value] ∗ 100.