Optimum Conditions and LC-ESI-MS Analysis of Phenolic Rich Extract from Eucalyptus marginata L. under Maceration and Ultrasound-Assisted Extraction Methods Using Response Surface Methodology

Table 1

The operating conditions according to an experiment plan.

N	X1 ME t (min)	X1UAE t (min)	X2 T (°C)	X3 R (ml/g)	X4 ethanol (%)
1	30	10	25	20	20
2	30	10	25	20	60
3	30	10	25	40	20
4	30	10	25	40	60
5	30	10	75	20	20
6	30	10	75	20	60
7	30	10	75	40	20
8	30	10	75	40	60
9	90	50	25	20	20
10	90	50	25	20	60
11	90	50	25	40	20
12	90	50	25	40	60
13	90	50	75	20	20
14	90	50	75	20	60
15	90	50	75	40	20
16	90	50	75	40	60
17	60	30	50	30	40
18	60	30	50	30	40
19	60	30	50	30	40
20	17.58	17.2	50	30	40
21	102.42	58.2	50	30	40
22	60	30	14.65	30	40
23	60	30	85.35	30	40
24	60	30	50	15.9	40
25	60	30	50	44.1	40
26	60	30	50	30	16.56
27	60	30	50	30	96.56

ME, maceration extraction; UAE, ultrasound-assisted extraction; t, extraction time expressed in min; T, temperature expressed in °C; R, solvent-solid ratio expressed in mg/g.