Optimum Conditions and LC-ESI-MS Analysis of Phenolic Rich Extract from Eucalyptus marginata L. under Maceration and Ultrasound-Assisted Extraction Methods Using Response Surface Methodology

Table 2

The selected factors and their levels in the CCD design.

	Factors	Unit	Symbol	Factor levels
				−1	0	1
ME	Extraction time	min	X1	30	60	90
	Temperature	°C	X2	25	50	75
	Solvent-to-material ratio	ml/g	X3	20	30	40
	Ethanol	%	X4	20	40	60
UAM	Extraction time	min	X1	10	30	60
	Temperature	°C	X2	20	50	75
	Solvent-to-material ratio	ml/g	X3	20	30	40
	Ethanol	%	X4	20	40	60

ME, maceration extraction; UAM, ultrasound-assisted extraction.