Optimum Conditions and LC-ESI-MS Analysis of Phenolic Rich Extract from Eucalyptus marginata L. under Maceration and Ultrasound-Assisted Extraction Methods Using Response Surface Methodology

Table 4

Phenolic compounds detected in Eucalyptus marginata L. leaves optimum extraction condition extracts.

N	Compounds	Formula	Molecular mass	[M-H]^− m/z	Retention time (min)	Maceration (µg/g DW)	Ultrasound (µg/g DW)
1	Quinic acid	C7H12O6	192	191	1.750	1.65 ± 0.02^a	ND
2	Gallic acid	C7H6O5	170	169	2.627	12.17 ± 0.06^a	27.77 ± 0.06^b
3	Protocatechuic acid	C7H6O4	154	153	8.617	ND	37.66 ± 0.04^b
4	p-Coumaric acid	C9H8O3	164	163	16.217	4.67 ± 0.06^a	ND
5	trans-Ferulic acid	C10H10O4	194	193	19.150	0.12 ± 0.03^a	ND
6	Hyperoside (quercetin-3-O-galactoside)	C21H20O12	464	463	20.744	96.47 ± 0.08^a	39.19 ± 0.03^b
7	Rutin (quercetin-3-O-rutinoside)	C27H30O16	610	609	21.742	0.23 ± 0.06^a	0.08 ± 0.09^b
8	Quercetrin (quercetin-3-O-rhamnoside)	C21H20O11	448	447	24.147	181.10 ± 0.05^a	150.78 ± 0.02^b
9	Naringin (naringenin-7-O-neohesperidoside)	C27H32O14	580	579	24.246	19.00 ± 0.05^a	8.80 ± 0.07^b
10	Salviolinic acid	C7H6O3	138	137	26.643	0.74 ± 0.04^a	0.75 ± 0.05^a
11	trans-Cinnamic acid	C9H8O2	148	147	28.794	7.11 ± 0.07^a	21.99 ± 0.01^b
12	Quercetin	C15H10O7	302	301	29.118	3.21 ± 0.01^a	2.06 ± 0.02^b
13	Naringenin	C15H12O5	272	271	31.478	1.23 ± 0.09^a	1.03 ± 0.06^b

Concentration expressed as µg/g of DW. ND, not detected; DW, dry weight. Results are expressed as mean ± standard deviation of three determinations. Means with different letters in the same line were significantly different at p < 0.05.