Steroid Hormone Exposure as a Potential Hazard in Milk Consumers: A Significant Health Challenge in Iran

Table 1

Linearity range and quantification limits for steroid hormones analyses using the ELISA method.

Steroid hormones	Range	Linear equation^†	r^2	Limit of quantification
17β-Estradiol (pg/ml)	20–2000 pg/ml	Y = −4.0453X^2 + 0.6277X + 3.2752	0.9948	8.68 pg/ml
Progesterone (ng/ml)	0.2–40 ng/ml	Y = −1.1442X^2 + 0.0356X + 1.726	0.9954	0.05 ng/ml
Hydroxyprogesterone (ng/ml)	0.2–20 ng/ml	Y = −0.0322X^2 − 0.8332X + 1.4682	0.9892	0.05 ng/ml

^†The absorbance changes (at 450 nm) were plotted relative to the logarithmic concentrations of each hormone.