Steroid Hormone Exposure as a Potential Hazard in Milk Consumers: A Significant Health Challenge in Iran

Table 2

Recoveries of steroid hormones in spiked milk samples.

Hormones	Hormone spiked	Hormone sample	Hormone found	Recovery (%)	RSD (%)^†
17β-Estradiol (pg/ml)	25	198	197	88.3	5.4
	50	198	413	92.1	4.4
	500	198	659	94.4	4.8
Progesterone (ng/ml)	5	4.4	8.9	94.6	6.1
	10	4.4	14.1	97.9	5.5
	20	4.4	21.1	86.4	5.8
Hydroxyprogesterone (ng/ml)	1.5	0.13	1.5	93.7	4.6
	3	0.13	2.7	86.2	5.7
	6	0.13	5.6	91.3	3.9

^†Relative standard deviation (RSD) was obtained from triplicate tests.