Variation in Phenolic, Mineral, Dietary Fiber, and Antioxidant Activity across Southern Tunisian Pearl Millet Germplasm
Table 5
Flavonoid compounds (µg·g−1 DM) identified in the methanol extracts of grains of millet genotypes.
Quercetin
Kaempferol
Silymarin
Apigenin
Cirsiliol
Hyperoside
Med.AG1.3
1.12 ± 0.53 a
0.22 ± 0.03 b
1.78 ± 0.31 cd
0.24 ± 0.07 b
3.68 ± 2.02 c
0.31 ± 0.26 b
Med.AG2.4
0.58 ± 0.10 b
0.26 ± 0.02 b
1.83 ± 1.22 cd
0.19 ± 0.03 bc
5.51 ± 1.04 abc
—
Med.AG3.1
0.60 ± 0.10 b
0.52 ± 0.10 a
_d
0.24 ± 0.07 b
4.85 ± 1.00 abc
—
Med.AG4.5
0.58 ± 0.30 b
0.18 ± 0.02 b
4.44 ± 2.00 a
0.14 ± 0.03 c
3.36 ± 2.00 c
0.18 ± 0.07 b
Med.AG4.6
0.46 ± 0.20 b
0.18 ± 0.02 b
3.71 ± 1.82 ab
0.19 ± 0.02 bc
3.90 ± 2.00 bc
0.05 ± 0.02 b
Jer.AG5.2
0.49 ± 0.30 b
0.24 ± 0.10 b
2.04 ± 1.00 bc
0.38 ± 0.02 a
3.34 ± 1.00 c
4.28 ± 2.00 a
Jer.AG6.1
0.49 ± 0.08 b
0.21 ± 0.02 b
_d
0.24 ± 0.04 b
4.94 ± 2.00 abc
—
Jer.AG6.2
0.46 ± 0.06 b
0.16 ± 0.04 b
0.55 ± 0.20 cd
0.15 ± 0.02 bc
6.70 ± 2.00 ab
—
Jer.AG7.1
0.46 ± 0.20 b
0.15 ± 0.01 b
_d
0.18 ± 0.08 bc
7.09 ± 1.00 a
—
Jer.AG7.2
0.53 ± 0.20 b
0.16 ± 0.05 b
0.65 ± 0.21 cd
0.16 ± 0.05 bc
2.66 ± 0.57 c
—
R²
0.459
0.855
0.771
0.733
0.548
0.86
CV
34.28998476
51.02691864
105.0947844
32.83744084
32.27193126
32.27193126
ANOVA
ns
Chromatographic analysis parameters
Mw
302
286
482
270
330
464
Ionization form
[M-H]-, [2M-H]-
[M-H]-, [2M-H]-
[M-H]-, [2M-H]-
[M-H]-, [2M-H]-
[M-H]-
[M-H]-, [2M-H]-
m/z
301
285
481
269
329
463
Retention time
31.999
32.010
33.848
34.624
35.353
24.308
LOD++ (ppm)
0.085
0.181
0.030
0.821
0.143
0.887
LOQ++ (ppm)
0.258
0.548
0.090
2.489
0.432
2.688
Values are averages ± SD (n = 3). Letters a–e denote statistical differences between assessed genotypes using Duncan’s multirange mean comparison test (α = 0.05). One-way ANOVA results revealing significant differences between genotypes are presented according to P values as follows: ns: ; 0.01 ≤ P < 0.05; 0.001 ≤ P < 0.01; and . ++ The limit of detection LOD = 3.3σ / S, and the limit of quantification LOQ = 10σ / S; σ is the standard deviation of the response, and S is the slope of the calibration curve.
