Validation of a ¹H-NMR Spectroscopy Quantitative Method to Quantify Trimethylamine Content and K-Index Value in Different Species of Fish

Figure 1

¹H-NMR (400.13 MHz) spectrum of red mullet; (B) ATP, ADP, and AMP contribute to the formation of peak at 8.27 ppm, adn IMP, HxR, and Hx appear as a singlet, respectively, at 8.57 ppm, 8.33 ppm, and 8.21 ppm. Signals from TMAO and N-TMA are, respectively, at 3.27 and 2.91 ppm.