Validation of a ¹H-NMR Spectroscopy Quantitative Method to Quantify Trimethylamine Content and K-Index Value in Different Species of Fish

Table 2

Recovery of TMA during extraction and accuracy of methods in spiked samples.

European sea bass (Dicentrarchus labrax)
TMA^a (μg/mL)	Recovery^b (%)		Bias^c (%)		RSD^d (%)		RSDH^e (%)		Horrat^f		Accuracy^g (%)
	UV	NMR	UV	NMR	UV	NMR	UV	NMR	UV	NMR	UV	NMR
20 (0.002)	100.9 (1.1)	99.4 (2.3)	0.9	-0.6	5.0	5.5	9.7	9.7	0.5	0.6	5.6	5.6
40 (0.003)	98.9(0.9)	100.3 (0.7)	−1.1	0.3	4.9	5.3	8.7	8.7	0.6	0.6	5.4	5.6
60 (0.003)	100.3 (0.5)	100.5(0.5)	0.3	0.5	4.9	5.2	8.2	8.2	0.7	0.6	5.5	5.5
Atlantic mackerel (Scomber scombrus)
TMA^a (μg/mL)	Recovery^b (%)		Bias^c (%)		RSD^d (%)		RSDH^e (%)		Horrat^f		Accuracy^g (%)
	UV	NMR	UV	NMR	UV	NMR	UV	NMR	UV	NMR	UV	NMR
20 (0.007)	96.6 (3.3)	98.1 (2.8)	−3.5	−1.9	5.3	5.6	9.7	9.7	0.5	0.6	6.4	5.9
40 (0.007)	98.8 (1.7)	101.1 (2.0)	1.2	1.1	5.5	5.7	8.7	8.7	0.6	0.7	5.6	5.8
60 (0.005)	99.8 (0.8)	99.3 (1.4)	0.2	0.7	5.7	5.4	8.2	8.2	0.7	0.7	5.6	5.5