Validation of a 1H-NMR Spectroscopy Quantitative Method to Quantify Trimethylamine Content and K-Index Value in Different Species of Fish
Table 3
TMA content in red mullet and bogue fish.
Red mullet (Mullus barbatus)
# Fish sample
TMA (mg/100 g)
RSD (%)a
RSDH (%)b
Horratc
UV
NMR
UV
NMR
UV
NMR
UV
NMR
1 (T1)
0.14 (0.04)
0.16 (0.02)
6.9
7.4
10.1
10.3
0.7
0.7
2 (T2)
0.29 (0.1)
0.27 (0.02)
3.4
4.3
9.2
9.2
0.4
0.5
3 (T4)
3.80 (0.5)
2.86 (0.2)
6.1
3.3
6.1
6.2
1.0
0.5
4 (T7)
18.87 (3.6)
11.95 (2.4)
3.6
3.8
4.8
5.2
0.7
0.7
5 (T9)
26.3 (3.9)
19.26(1.2)
4.0
1.2
4.7
4.8
0.9
0.3
6 (T11)
37.54 (1.8)
25.11 (1.1)
2.2
2.0
4.4
4.7
0.5
0.4
Bogue fish (Boops boops)
# Fish sample
TMA (mg/100 g)
RSD (%)a
RSDH (%)b
Horratc
UV
NMR
UV
NMR
UV
NMR
UV
NMR
1 (T1)
0.45 (0.16)
0.33 (0.05)
9.2
6.6
8.7
9.2
1.0
0.7
2 (T2)
1.02 (0.07)
0.66 (0.07)
3.2
4.7
7.6
8.0
0.4
0.6
3 (T4)
6.6 (0.05)
6.8 (0.4)
1.3
2.7
5.7
5.7
0.2
0.5
4 (T7)
16.7 (1.3)
11.4 (0.9)
3.4
3.4
5.0
5.3
0.7
0.6
5 (T9)
17.7 (3.6)
12.8 (1.9)
2.7
3.0
5.2
5.2
0.5
0.6
6 (T11)
21.5 (4.3)
20.03 (4.3)
3.9
4.7
4.7
5.1
0.8
0.9
aRelative standard deviation. bAcceptable relative standard deviation according to Horwitz’s formula for intralaboratory studies. cHorrat = RSD measured/RSD Horwitz predicted.
