Species Identification of Bovine Bone Marrow from Nonbovine Products Using Multiplex PCR Technology

Figure 2

Agarose gel electrophoresis of genomic DNA extracts and species-specific fragments by multiplex PCR. (a) Agarose gel electrophoresis of genomic DNA extracted from different animals’ bone marrows using an SDS alkaline lysis method. M: Marker; 1∼3: BBM listed as 2016BH001, 2016BH002, and 2016BH003; 4: donkey bone marrow; 5: pig bone marrow; 6: sheep bone marrow; 7: a mixture of DNA extracted from horse and deer bone marrow (1 : 1). (b) Agarose gel electrophoresis of species-specific fragments by multiplex PCR. 1: Marker; 2: a mixture of DNA extracted from three species (1 : 1 : 1); 3∼5: BBM listed as 2016BH001, 2016BH002, and 2016BH003; 6: donkey bone marrow; 7: pig bone marrow; 8: sheep bone marrow; 9: a mixture of DNA extracted from horse and deer bone marrow (1 : 1).

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