Species Identification of Bovine Bone Marrow from Nonbovine Products Using Multiplex PCR Technology

Figure 3

Specificity and sensitivity tests of the PCR assay using gel electrophoresis. (a) Specificity tests of the simplex-PCR assay using gel electrophoresis. M: Marker; 1: a mixture of DNA extracted from three species; 2∼4: bovine bone marrow listed as 2016BH001, 2016BH002, and 2016BH003; 5∼6: commercially available BBM; 7∼8: donkey bone marrow; 9: pig bone marrow; 10: sheep bone marrow; 11: deer bone marrow; 12: horse bone marrow; 13: negative control. (b) Specificity tests of the multiplex PCR assay using gel electrophoresis. M: Marker; 1: a mixture of DNA extracted from three species; 2∼4: bovine bone marrow listed as 2016BH001, 2016BH002, and 2016BH003; 5∼6: commercially available BBM; 7∼8: donkey bone marrow; 9: pig bone marrow; 10: sheep bone marrow; 11: deer bone marrow; 12: horse bone marrow; 13: negative control. (c) Sensitivity tests of the PCR assay using gel electrophoresis. M: Marker; 1: DNA extracts of BBM samples diluted to100 ng/μL; 2 : 10 ng/μL; 3: 1 ng/μL; 4 : 100°pg/μL; 5 : 10°pg/μL; 6 : 1°pg/μL; 7 : 0.1°pg/μL; 8 : 0.01°pg/μL; and 9: negative control.

(a)

(b)

(c)