Effect of Cholesterol to Vitamin D3 and Span 60 to Tween 60 Ratios on the Characteristics of Niosomes: Variable Optimization Using Response Surface Methodology (RSM)

Table 1

Variables and levels and responses used in RSM.

Run	Factor 1	Factor 2	Responses
	SP60 : TW60	CSL : VD3	Size (nm)	Span	EE (%)	Stability (%)
1	60 : 40	0 : 100	76.13	4.19	1.12	0.462
2	100 : 00	50 : 50	335.77	0.59	81.14	0.456
3	88.28 : 11.72	14.65 : 85.35	142.10	3.17	21.42	0.467
4	31.72 : 68.28	85.36 : 14.64	136.33	1.07	23.25	0.576
5	88.28 : 11.72	85.36 : 14.64	443.33	0.25	60.46	0.422
6	60 : 40	50 : 50	95.50	0.23	89.56	0.455
7	60 : 40	50 : 50	99.90	0.15	90.36	0.447
8	60 : 40	100 : 0	332.10	1.16	2.63	0.379
9	31.72 : 68.28	14.65 : 85.35	71.23	1.67	71.13	0.611
10	20 : 80	50 : 50	77.50	0.09	91.17	0.656
11	60 : 40	50 : 50	97.50	0.16	90.56	0.441

SP60: Span 60, TW60: Tween 60, CSL: cholesterol, VD3: vitamin D3, and EE: encapsulation efficiency.