Effect of Cholesterol to Vitamin D3 and Span 60 to Tween 60 Ratios on the Characteristics of Niosomes: Variable Optimization Using Response Surface Methodology (RSM)

Table 2

Estimated coefficients of multiple determinations (R²) for particle size using coded values.

Source	Sum of squares	df	Mean square	F-value	value
Model	1.73 × 10^5	5	34592.0	1295.47	<0.0001	Significant
A-A	69026.72	1	69026.7	2585.05	<0.0001
B-B	66307.10	1	66307.1	2483.2	<0.0001
AB	13939.74	1	13939.7	522.04	<0.0001
A^2	15694.67	1	15694.7	587.77	<0.0001
B^2	14954.34	1	14954.3	560.04	<0.0001
Residual	133.51	5	26.7
Lack of fit	123.80	3	41.2	8.5	0.107	Not significant
Pure error	9.71	2	4.8
Cor total	1.73 × 10^5	10

R ²: 0.9992; adjusted R²: 0.9985; predicted R²: 0.9948.