Enhancement of Vitamin K2 Efflux in Bacillus subtilis Natto via a Potential Protein Receptor for Increased Yield

Figure 2

(a) Western blot analysis of BslA proteins. M, protein marker; S, supernatant; P, precipitate; 1, E. coli Rosetta with pET-28a-BslA, 30°C, 180 rpm, 4 h; 2, E. coli Rosetta with pET-28a-BslA, IPTG, 30°C, 180 rpm, 4 h. (b) Elution effect of BslA on Ni-NTA purification. (1–4) The eluent of the first column volume to the eluent of the fourth column volume.

(a)

(b)