Detection of Viable Zygosaccharomyces rouxii in Honey and Honey Products via PMAXX-qPCR

Table 1

Comparison of the effect of PMA and PMAXX on detection of viable Z. rouxii.

Dyes or compounds	Concentration (μM)	Ct^a (viable cells)	Ct^b (dead cells)	ddCt^c
PMA	0.00; 19.80; 27.61; 31.50; 39.22; 58.25; 76.92; 113.21; 148.15	18.193 ± 0.054; 18.438 ± 0.213; 19.502 ± 0.100; 19.081 ± 0.101; 21.353 ± 0.051; 19.980 ± 0.079; 19.581 ± 0.023; 20.076 ± 0.072; 19.764 ± 0.090	19.824 ± 0.139; 25.085 ± 0.560; 25.241 ± 0.269; 26.062 ± 0.019; 26.908 ± 0.007; 27.839 ± 0.133; 27.021 ± 0.020; 27.394 ± 0.026; 27.612 ± 0.330	1.29; 7.09; 5.54; 19.23; 14.32; 9.99; 14.25; 12.24
PMAXX	0.00; 2.00; 2.80; 3.99; 23.72; 27.61; 31.50; 39.22; 58.25; 76.92; 113.21; 148.15	18.193 ± 0.054; 21.469 ± 0.064; 21.892 ± 0.007; 22.015 ± 0.220; 22.279 ± 0.164; 22.235 ± 0.085; 21.184 ± 0.256; 20.938 ± 0.247; 21.276 ± 0.179; 20.987 ± 0.380; 20.496 ± 0.025; 28.616 ± 0.035	19.824 ± 0.139; 22.581 ± 0.178; 22.853 ± 0.601; 24.142 ± 0.054; 28.168 ± 0.256; 28.249 ± 0.061; 29.130 ± 0.094; 29.888 ± 0.152; 30.483 ± 0.223; 33.171 ± 0.254; 32.526 ± 0.014; 29.043 ± 0.104	8.52; 10.20; 16.50; 34.09; 34.05; 27.83; 27.63; 32.86; 37.29; 29.25; 96.09

Ct^a and Ct^b: mean Ct value ± standard deviation (SD); ddCt^c = dCt (dead cells) × dCt (viable cells); dCt (dead cells) = Ct (dead cells with dye)–Ct (dead cells without dye); dCt (viable cells) = Ct (viable cells with dye)–Ct (viable cells without dye).