Detection of Viable Zygosaccharomyces rouxii in Honey and Honey Products via PMAXX-qPCR

Table 2

Detection results of viable Z. rouxii cells in honey samples by PMAXX-qPCR, qPCR, and plate count.

Sample no.	^eCt, PMAXX-qPCR	^fCt, qPCR	Plate count result (CFU/ml)
1	^dN	28.346	0
2	36.337	33.303	0
3	36.465	25.272	0
4	^dN	31.045	0
5	35.764	33.030	0
6	37.855	30.490	0
7	36.310	27.390	0
8	^dN	35.731	0
9	38.611	36.180	0
10	34.134	24.198	0
11	^dN	38.001	0
12	35.302	29.252	0
13	^dN	29.252	0
14	^dN	31.223	0
15	^dN	34.472	0
16	^dN	34.437	0
17	^dN	33.082	0
18	^dN	24.208	0
PC	—	20.673	—
NC	^dN	^dN	0

^dN, undetermined; ^eCt, PMAXX-qPCR: Ct value of qPCR with the treatment of PMAXX; ^fCt, qPCR: Ct value of qPCR without the treatment of PMAXX.