Simultaneous Determination of B₁, B₂, B₃, B₆, B_9, and B₁₂ Vitamins in Premix and Fortified Flour Using HPLC/DAD: Effect of Detection Wavelength

Table 1

Summary of reported methods for simultaneous determination of B vitamins in multivitamin supplements, energy drinks, and pharmaceuticals.

Sample (reference)	Method	Stationary phase	Mobile phase	Elution time	Sequence of elution time (detection wavelength nm)
Pharmaceuticals [12]	HPLC-UV	C18	Gradient A: 10%v/v propanol in 0.01 M H3PO4 with 0.03 M sodium dodecyl sulfate. B: same as A but 60% v/v propanol	12 min	B2(270), B3(264), B9(264), B6(290), and B1(245)
Baby foods [13]	HPLC-UV	Amide C16	Gradient A: 0.01 M KH2PO4 (pH 6). B: acetonitrile	30 min	B3(266), B6(326), B1(266), B9(266), B12(361), and B2(266)
Multivitamin dietary supplement [14]	LC-UV/MS	C18	Gradient A: 0.1% formic acid in water. B:0.1% formic acid in acetonitrile	18 min	B1(260), B3(260), B6(292), B9(280), and B2(270)
Pharmaceuticals and premix [15]	HPLC-DAD	C18	Gradient A: 0.6% H3PO4 (pH 1.7) B: acetonitrile	30 min	B1(244), B6(292), B9(297), and B2(270)
Infant formula, cereal, and multivitamin pill [16]	HPLC-DAD	C30	Gradient A: 0.02 M KH2PO4 (pH 3) B: 50%v/v in 0.02 M KH2PO4 in acetonitrile	50 min	B1, B3, B6, B9, B12, and B2 (275 for all)
Energy drink [17]	HPLC-DAD	C18	Gradient A: methanol. B:0.05 M NaH2PO4 and 0.05 M hexanesulfonic acid (pH 3)	30 min	B3(260), B6(290), B1(245), B9(280), B12(207, 360), and B2(450)
Multivitamin dietary supplement [18]	HPLC-UV	C18	A: 0.005 M sodium hexanesulfonic acid, 0.02 M H3PO4, 0.016 M triethylamine (pH 3.0). B: acetonitrile 75:25 (v/v)	60 min	B3, B6, B1, B9, B2, B12 (210 for all)
Veterinary premix [19]	HPLC-UV	C18	Gradient A: 0.025 M KH2PO4 and 0.005 M sodium hexanesulfonate (pH 4). B: 0.005 M sodium hexanesulfonate in methanol	25 min	B6, B1, B2 (278), and B12 (361)
Plasma and urine [20]	HPLC-UV	C18	Gradient A: 0.05 M KH2PO4 and 0.005 M sodium heptanesulfonate (pH 3). B: acetonitrile	22 min	B3, B6, B2, and B1 (240)