Simultaneous Determination of B₁, B₂, B₃, B₆, B_9, and B₁₂ Vitamins in Premix and Fortified Flour Using HPLC/DAD: Effect of Detection Wavelength

Table 2

Gradient elution programme.

Time	% A (0.03% TFA in water)	% B (acetonitrile)
0	100	0
2	100	0
4.5	83	17
9.5	83	17
9.6	100	0
13.5	Equilibration