Simultaneous Determination of B₁, B₂, B₃, B₆, B_9, and B₁₂ Vitamins in Premix and Fortified Flour Using HPLC/DAD: Effect of Detection Wavelength

Table 4

Precision (RSD % for peak area) under repeatability conditions (n = 6). Intermediate precision (n = 7).

Vitamin		265 nm	361 nm	280 nm	210 nm
B1	Repeatability	0.78		1.27	2.14
	Intermediate	0.79		1.55	2.25
B2	Repeatability	0.75	1.79	1.70	0.75
	Intermediate	1.33	2.54	2.35	1.33
B3	Repeatability	1.16		1.83	1.95
	Intermediate	0.63		2.30	1.86
B6	Repeatability	1.76		1.15	0.53
	Intermediate	2.22		1.37	0.70
B9	Repeatability	1.08	2.31	0.84	1.23
	Intermediate	1.38	2.44	0.92	1.39
B12	Repeatability	0.86	1.30	0.86	1.05
	Intermediate	0.74	1.45	0.89	1.35