Simultaneous Determination of B₁, B₂, B₃, B₆, B_9, and B₁₂ Vitamins in Premix and Fortified Flour Using HPLC/DAD: Effect of Detection Wavelength

Table 5

Linearity of calibration curves, sensitivity, limit of detection (LOD), and limit of quantification (LOQ) for vitamins.

Vitamin	λ (nm)	Slope (sensitivity)	Y-intercept	R ^2	Linear range (μg/mL)	LOD (μg/mL)	LOQ (μg/mL)
B1	265	0.7242	−0.0231	0.9999	0.117-14.9	0.179	0.596
	280	0.3122	−0.0159	0.9999		0.175	0.585
	210	0.4023	−0.0553	0.9970		0.854	2.847
B2	265	1.4615	0.0464	0.9999	0.122-7.73	0.066	0.219
	361	0.4485	0.0006	1.0000		0.046	0.154
	280	0.9746	0.0132	1.0000		0.048	0.159
	210	0.8165	0.6794	0.9645		1.842	6.141
B3	265	0.6777	0.0214	0.9998	0.113-14.4	0.243	0.811
	280	0.0690	−0.0050	0.9995		0.371	1.236
	210	1.0842	0.0762	0.9970		0.882	2.942
B6	265	0.1616	0.0416	0.9995	0.107-13.6	0.354	1.181
	280	0.6143	0.0792	0.9978		0.708	2.360
	210	1.2621	0.0443	1.0000		0.081	0.270
B9	265	0.6889	−0.0407	0.9998	0.158-10	0.181	0.605
	361	0.2270	−0.0126	0.9997		0.196	0.654
	280	1.0625	−0.0533	0.9998		0.155	0.516
	210	1.0771	−0.0504	0.9997		0.187	0.622
B12	265	0.1813	0.0089	0.9999	0.156-20.6	0.206	0.685
	361	0.3645	0.0182	0.9999		0.197	0.657
	280	0.2050	0.0104	0.9999		0.206	0.686
	210	1.0820	0.0225	1.0000		0.067	0.223