Research Article
Simultaneous Determination of B1, B2, B3, B6, B9, and B12 Vitamins in Premix and Fortified Flour Using HPLC/DAD: Effect of Detection Wavelength
Table 6
Determination of B vitamins in premix and the percentage of measured per claimed values (M/C %).
| | Vitamin | λ (nm) | Labeled (mg/kg) | Determined (mg/kg) | RSD (%) | (M/C %) |
| | B1 | 265 | 11592 | 14374 | 3.6 | 124 | | 280 | | 14374 | 3.3 | 124 | | 210 | | 14490 | 3.9 | 125 |
| | B2 | 265 | 14400 | 14688 | 3.6 | 102 | | 361 | | 13248 | 16.1 | 92 | | 280 | | 14688 | 3.7 | 102 | | 210 | | 12240 | 4.5 | 85 |
| | B3 | 265 | 140000 | 138600 | 3.4 | 99 | | 280 | | 133000 | 4.5 | 95 | | 210 | | 144200 | 3.7 | 103 |
| | B6 | 265 | 14480 | 13322 | 3.9 | 92 | | 280 | | 13177 | 3.9 | 91 | | 210 | | 13032 | 3.9 | 90 |
| | B9 | 265 | 6064 | 970 | 29.3 | 16 | | 361 | | 849 | 39.5 | 14 | | 280 | | 1031 | 31.0 | 17 | | 210 | | 970 | 33.3 | 16 |
| | B12 | 265 | 30.50 | 47.88 | 5.1 | 157 | | 361 | | 42.70 | 16.8 | 140 | | 280 | | 44.53 | 4.7 | 146 | | 210 | | 48.19 | 7.6 | 158 |
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The labeled values were obtained from the Ministry of Health–MOH, Jordan (2021). |
