Simultaneous Determination of B₁, B₂, B₃, B₆, B_9, and B₁₂ Vitamins in Premix and Fortified Flour Using HPLC/DAD: Effect of Detection Wavelength

Table 7

Determination of B vitamins in fortified flour, and the percentage of measured per claimed values (M/C %). The claimed values were calculated depending on the mixing proportions of premix and flour.

Vitamin	λ (nm)	(M/C %)	RSD (%)
B1	265	91	1.7
	280	91	1.7
	210	103	3.9
B2	265	61	8.3
	361	62	7.5
	280	61	8.7
	210	95	13.4
B3	265	75	2.1
	280	75	3.2
	210	72	6.2
B6	280	118	6.8
	210	139	5.6
B9	265	18	4.5
	361	15	8.8
	280	17	4.7
	210	17	5.0
B12	265	135	6.8
	361	105	3.3
	280	109	5.6
	210	120	5.9