Characterization of Two Wheat-Derived Glycoside Hydrolase Family-10 Xylanases Resistant to Xylanase Inhibitors

Figure 3

Analysis of recombinant fusion proteins through SDS-PAGE. SDS-PAGE gel of recombinant proteins: lane M-low MW protein markers; lane 1-crude supernatant of E. coli cells without IPTG induction; lane 2-crude EXY 4 supernatant of E. coli cells with IPTG induction; lane 3-purified EXY 4 proteins; lane 4-crude supernatant of E. coli cells without IPTG induction; lane 5-crude EXY4 supernatant of E. coli cells with IPTG induction; and lane 6-purified EXY 3 proteins.