Stability and Survivability of Alginate Gum-Coated Lactobacillus rhamnosus GG in Simulated Gastrointestinal Conditions and Probiotic Juice Development

Table 3

Physicochemical analysis of free and encapsulated L. rhamnosus-treated orange juice.

Parameters	Treatments	0 Day	7 Day	14 Day	21 Day
pH	T0	2.93 ± 0.02^abc	2.91 ± 0.03^bcd	2.87 ± 0.03^def	2.85 ± 0.01^bcd
	T1	2.94 ± 0.03^ab	2.84 ± 0.01^cdef	2.70 ± 0.02^g	2.58 ± 0.02^h
	T2	2.94 ± 0.03^ab	2.83 ± 0.04^cdef	2.80 ± 0.04^f	2.76 ± 0.03^fg
	T3	2.95 ± 0.01^a	2.90 ± 0.02^cde	2.87 ± 0.05^def	2.82 ± 0.03^ef
Total sugars (% brix)	T0	12.12 ± 0.05^a	12.00 ± 0.05^b	11.67 ± 0.04^c	11.00 ± 0.07^de
	T1	12.10 ± 0.08^ab	10.66 ± 0.06^f	8.40 ± 0.06^i	7.90 ± 0.06^j
	T2	12.11 ± 0.06^ab	11.04 ± 0.07^d	10.15 ± 0.08^g	9.50 ± 0.05^h
	T3	12.11 ± 0.05^a	11.70 ± 0.09^c	11.07 ± 0.03^d	10.90 ± 0.04^e
Acidity (%)	T0	0.96 ± 0.02^hi	1.04 ± 0.01^efg	1.10 ± 0.02^cde	1.15 ± 0.01^c
	T1	0.94 ± 0.01^i	1.12 ± 0.04^cd	1.24 ± 0.03^b	1.35 ± 0.02^a
	T2	0.95 ± 0.01^i	1.02 ± 0.03^fgh	1.07 ± 0.01^def	1.14 ± 0.01^c
	T3	0.95 ± 0.02^i	1.00 ± 0.05^ghi	1.03 ± 0.009^fgh	1.05 ± 0.01^efg

Mean values ± S.D. in a column having similar lettering are statistically nonsignificant at 5% confidence interval.
T₀ = control, plain juice; T₁ = free Lactobacillus rhamnosus; T₂ = sodium alginate (1.5%)-encapsulated L. rhamnosus; T₃ = sodium alginate (1.5%)- and xanthan gum (0.5%)-encapsulated L. rhamnosus.