Characterization and Antimicrobial Susceptibility Patterns of Listeria monocytogenes from Raw Cow Milk in the Southern Part of Ethiopia

Table 1

Primer sequences used in the mPCR reactions for the detection of 16S rRNA and virulence-associated genes in L. monocytogenes.

Target gene	Primer sequences (5′–3′)	Amplicon size (bp)
16S rRNA	F: CAGCAGCCGCGGTAATAC	938
	R: CTCCATAAAGGTGACCCT
hlyA	F: GCA​GTT​GCA​AGC​GCT​TGG​AGT​GAA	456
	R: GCA​ACG​TAT​CCT​CCA​GAG​TGA​TCG
PlcA	F: CTG​CTT​GAG​CGT​TCA​TGT​CTC​ATC​CCC​C	1484
	R: CAT​GGG​TTT​CAC​TCT​CCT​TCT​AC
actA	F: CGC​CGC​GGA​AAT​TAA​AAA​AAG​A	839
	R: ACG​AAG​GAA​CCG​GGC​TGC​TAG
prfA	F: CTG​TTG​GAG​CTC​TTC​TTG​GTG​AAG​CAA​TCG	1060
	R: AGC​AAC​CTC​GGT​ACC​ATA​TAC​TAA​CTC
iapA	F: ACA​AGC​TGC​ACC​TGT​TGC​AG	131
	R: TGA​CAG​CGT​GTG​TAG​TAG​CA

F: forward; R: reverse.