Journal of Food Quality / 2024 / Article / Tab 3 / Research Article
Determination of 60 Food-Borne Stimulant Drug Residues in Animal-Derived Foods by Solid-Phase Extraction Purification and Ultra-High-Performance Liquid Chromatography-Quadrupole/Orbitrap High-Resolution Mass Spectrometry Table 3 Recoveries and relative standard deviation (RSD) of the 60 food-borne stimulants (n = 6).
Compound Spiked level (μ g/kg) Milk Egg Beef Mutton Pork Average recoveries (%) RSD (%) Average recoveries (%) RSD (%) Average recoveries (%) RSD (%) Average recoveries (%) RSD (%) Average recoveries (%) RSD (%) Clenbuterol 0.2 77.6 5.33 72.0 1.50 79.6 6.92 75.6 8.88 75.4 0.29 0.4 95.3 2.82 87.7 5.62 91.2 5.43 85.4 5.36 79.6 5.51 2 93.5 6.35 95.7 4.69 93.4 4.44 87.0 8.51 90.0 0.80 Salbutamol 0.2 101.1 8.21 110.8 2.51 104.9 5.96 104.2 3.39 110.4 2.71 0.4 107.2 3.58 105.7 8.63 110.7 3.81 110.3 8.03 109.7 5.06 2 103.8 0.13 109.2 5.19 108.7 2.23 104.8 5.75 103.7 3.16 Ractopamine 0.1 104.0 3.71 111.8 8.09 110.1 2.70 110.2 6.06 103.9 2.98 0.2 102.2 5.04 106.2 0.73 103.4 8.07 104.3 0.26 106.4 5.90 1 107.8 6.67 110.4 2.42 104.6 3.81 106.8 1.16 104.2 6.26 Terbutaline 0.2 86.2 6.33 92.4 6.62 98.9 8.13 96.3 6.28 92.6 2.43 0.4 103.1 4.77 90.1 0.66 88.8 1.49 110.2 5.89 96.4 1.59 2 95.3 2.79 98.1 3.97 105.8 0.40 93.5 7.95 89.3 0.65 Fenoterol 0.1 105.4 4.49 105.2 4.50 107.2 7.53 105.9 4.74 107.8 6.04 0.2 110.4 1.89 109.5 1.21 105.8 4.33 107.6 5.94 102.4 3.07 1 102.5 1.52 110.3 3.53 110.1 1.16 103.5 1.38 104.4 0.63 Tulobuterol 0.1 107.0 7.00 101.1 4.77 101.4 1.30 107.6 1.34 108.7 1.96 0.2 102.5 3.16 110.3 1.15 107.5 1.48 107.5 8.47 109.4 5.14 1 103.8 2.31 106.9 4.29 103.3 5.30 106.9 3.26 107.9 6.54 Penbutolol 0.2 93.7 8.43 111.7 7.65 107.4 6.02 90.7 1.24 95.3 1.72 0.4 106.2 4.56 108.3 4.26 99.6 5.16 88.1 1.89 82.2 2.67 2 105.0 0.27 86.0 6.81 102.9 8.93 95.8 5.62 103.4 1.67 Cimaterol 0.2 88.0 6.45 91.6 4.08 88.1 5.16 95.1 8.64 87.9 2.40 0.4 92.8 4.05 83.0 7.13 85.0 5.30 80.2 6.07 81.2 8.70 1 98.3 6.14 85.2 8.23 83.6 4.48 94.4 1.67 86.5 8.88 Salmeterol 0.1 108.2 7.33 95.4 5.46 106.7 8.36 97.7 1.22 101.5 2.07 0.2 108.8 2.99 95.5 1.21 89.2 3.06 102.7 7.14 86.7 1.15 1 107.0 1.48 107.8 5.66 84.0 3.37 107.3 2.64 89.6 8.97 Atenolol 0.2 103.0 1.36 97.8 8.35 101.9 3.21 108.7 4.18 92.0 4.35 0.4 106.5 0.47 102.2 7.53 100.8 4.59 110.7 1.10 88.6 5.25 2 104.6 7.24 101.1 2.42 90.8 6.14 106.3 5.33 107.2 5.81 Metoprolol 0.1 109.8 5.22 105.5 5.66 92.6 2.43 94.0 2.81 108.8 8.12 0.2 110.8 1.37 108.3 2.90 105.4 6.63 99.4 0.42 99.1 6.22 1 98.9 7.47 109.3 0.66 104.4 5.74 90.0 3.92 105.2 2.69 Clenproperol 0.2 93.0 6.09 93.8 6.22 105.8 5.03 101.2 6.56 101.7 4.44 0.4 108.9 4.72 102.0 3.16 97.8 6.68 100.2 5.40 100.5 2.86 2 98.2 1.39 96.4 5.16 97.1 1.61 101.8 7.18 107.4 4.33 Demethyl coclaurine 0.2 92.1 0.45 92.3 5.48 100.1 7.08 96.3 6.21 99.0 1.70 0.4 91.2 8.91 104.0 8.21 96.7 6.93 102.6 2.66 102.2 1.92 2 104.6 1.43 109.4 3.14 97.6 2.38 110.0 3.60 98.4 0.58 Tretoquinol 0.2 101.6 1.00 94.3 3.65 108.8 0.05 99.3 4.67 106.8 6.69 0.4 108.0 2.40 109.7 2.13 93.9 8.87 96.0 1.81 110.4 6.84 2 97.9 4.80 97.6 7.35 100.3 5.48 94.9 2.22 104.9 6.92 Propanolol 0.1 105.6 1.81 103.0 6.13 91.7 2.04 93.2 7.58 96.6 4.29 0.2 110.1 7.00 102.7 2.29 101.2 2.95 106.5 8.08 103.2 8.42 1 104.5 1.35 107.0 3.70 105.0 1.26 106.3 6.81 96.8 1.98 Meprednisone 0.2 104.3 5.84 91.8 4.14 104.9 0.83 91.9 6.36 87.2 5.97 0.4 110.3 4.57 93.3 5.53 89.6 1.85 83.8 1.15 111.0 3.59 2 101.0 0.77 92.1 8.88 99.1 2.56 104.4 7.34 84.7 7.45 Prednisolone 0.2 105.5 1.87 101.1 3.93 103.7 3.76 110.4 1.24 95.2 4.55 0.4 103.3 8.03 91.2 1.78 106.9 5.87 95.0 4.71 110.6 7.60 2 101.0 2.39 103.9 4.88 90.8 8.59 94.5 5.55 95.6 4.63 Methylprednisolone 0.2 97.2 5.42 93.1 0.74 110.0 2.10 86.6 0.38 95.0 4.55 0.4 103.7 7.07 104.7 6.40 86.2 1.60 98.8 3.38 107.9 3.91 2 103.6 1.82 99.4 2.98 96.1 6.74 87.1 4.49 87.5 7.62 Dexamethasone 0.2 84.6 7.14 105.5 4.87 101.8 4.01 107.2 2.03 84.7 1.76 0.4 92.7 8.77 110.9 7.19 102.6 8.40 85.1 4.53 101.0 1.57 2 92.8 3.73 105.6 2.94 93.4 0.61 103.0 8.01 97.9 8.49 Betamethasone 0.2 96.9 7.40 94.2 6.27 105.8 7.07 88.0 1.34 103.4 5.07 0.4 91.8 8.38 91.2 8.12 105.8 7.18 101.4 2.92 102.4 6.77 2 102.1 2.29 104.8 6.55 87.3 6.51 111.7 8.58 105.5 1.21 Beclomethasone 0.2 75.4 5.12 107.0 3.18 90.6 6.35 110.4 6.63 89.9 2.36 0.4 91.3 3.54 108.2 3.89 98.8 6.02 73.4 8.06 90.9 3.53 2 109.6 4.65 86.0 3.96 104.7 7.55 103.2 7.69 90.3 2.17 Fludrocortisone 0.2 78.0 8.61 100.7 5.27 78.0 2.83 78.7 1.23 75.7 7.74 0.4 99.4 3.56 81.1 1.58 77.0 5.23 95.2 2.68 87.5 3.61 2 93.6 5.33 74.2 6.99 95.6 5.07 73.7 8.26 87.8 6.44 Hydrocortisone 0.2 108.5 6.42 105.0 8.18 107.6 2.15 103.8 5.72 94.8 4.25 0.4 105.5 0.52 101.3 3.81 92.5 8.92 94.2 2.94 103.1 7.75 2 102.1 2.21 105.2 5.13 108.2 1.67 95.1 6.34 107.9 0.05 Cortisone 0.2 103.0 7.37 93.5 8.24 93.3 6.06 100.3 5.33 103.0 7.72 0.4 104.3 6.13 94.7 2.96 85.4 4.51 100.4 4.94 96.5 6.08 2 110.5 2.64 104.4 7.28 83.5 2.82 95.7 7.40 108.6 1.43 Zilpaterol 0.2 106.1 8.36 103.1 2.55 107.8 5.94 98.6 1.01 88.1 7.80 0.4 108.3 5.98 83.5 2.82 102.1 7.08 83.0 1.98 94.3 7.99 2 91.4 2.16 98.6 7.02 93.4 2.19 105.9 2.19 100.3 1.31 Stanozolol 0.2 108.7 8.66 107.6 0.23 104.5 5.47 95.6 3.62 104.4 2.88 0.4 91.1 2.01 82.0 5.14 107.2 0.60 106.9 2.84 102.6 0.45 2 109.6 6.51 103.9 0.87 101.2 2.96 86.6 0.43 106.3 6.83 Trenbolone 0.1 110.7 1.14 105.5 8.51 89.5 0.74 103.1 0.85 106.5 0.62 0.2 109.6 3.18 102.3 1.74 96.4 5.67 109.6 3.66 110.9 6.11 1 102.4 8.97 96.4 0.08 91.8 4.30 105.6 2.88 107.3 6.00 Metandienone 0.2 102.5 7.04 108.9 2.76 103.5 2.43 106.3 0.91 106.8 5.76 0.4 106.5 0.05 100.9 0.50 106.8 6.68 108.2 5.82 105.9 3.07 2 110.7 7.37 99.1 7.69 105.8 8.46 103.7 3.88 105.4 1.53 17-Methyltestosterone 0.2 110.5 1.06 99.7 4.98 110.6 0.62 110.0 1.04 106.3 6.01 0.4 108.9 2.01 110.9 3.76 109.6 4.07 96.9 0.05 110.6 7.46 2 102.7 5.03 104.9 5.43 89.8 3.29 101.8 6.36 93.3 7.74 Testosterone 0.2 106.1 3.83 110.6 2.29 92.8 7.90 98.8 3.06 93.9 1.74 0.4 102.4 8.08 105.7 6.90 101.5 5.73 96.0 0.42 99.2 2.97 2 104.8 7.98 102.1 4.58 94.8 6.50 91.6 7.82 108.4 7.00 Nandrolone 0.2 104.7 0.89 80.5 3.88 95.9 0.24 89.3 7.75 99.9 7.70 0.4 90.0 4.98 103.7 1.35 87.4 5.55 103.1 3.43 108.2 8.63 2 82.5 4.16 87.2 7.82 108.4 4.04 106.3 2.22 106.7 0.99 Testosterone propionate 0.2 101.3 2.20 95.6 6.40 102.2 3.62 101.8 5.48 103.4 7.01 0.4 107.8 1.20 104.5 6.68 98.2 7.89 98.9 4.87 107.2 7.48 2 93.2 3.42 88.2 8.58 94.6 5.51 109.2 8.74 110.9 3.09 Nandrolone 17-propionate 0.2 109.4 2.00 100.9 0.73 105.0 2.59 94.2 0.47 96.1 7.17 0.4 104.3 0.05 91.1 2.18 94.8 2.83 109.2 5.10 100.4 2.79 2 95.4 3.60 94.7 6.31 101.9 8.94 88.8 0.80 101.6 2.83 Boldenone 0.2 109.2 2.30 101.7 5.88 101.1 6.25 92.8 3.17 108.3 6.42 0.4 110.2 6.11 95.6 4.61 92.2 5.66 85.0 2.10 88.4 1.11 2 104.0 1.94 89.2 0.64 104.2 7.65 101.2 7.78 107.8 7.10 Nandrolone phenylpropionate 0.1 102.3 6.47 89.9 0.23 97.2 4.78 86.0 8.88 101.1 6.64 0.2 95.3 8.60 88.4 1.74 96.6 0.11 86.9 7.67 102.8 2.23 1 89.4 8.36 86.3 5.67 102.2 5.55 109.4 2.77 93.1 5.24 Dehydroepiandrosterone 0.2 103.2 6.17 108.1 8.35 101.6 4.22 109.1 1.38 107.6 0.54 0.4 105.3 4.88 97.0 5.95 101.7 4.51 96.5 3.22 98.8 0.55 2 93.1 6.26 95.7 5.94 93.4 5.47 103.2 6.35 91.4 3.96 Zeranol 0.2 108.1 1.73 104.7 7.09 94.3 5.42 104.3 7.45 81.8 0.93 0.4 92.8 7.24 100.9 0.74 103.3 1.28 95.5 7.34 88.2 7.13 2 81.5 2.82 86.4 1.15 100.0 3.83 100.8 7.63 94.9 7.50 Beta-zearalanol 0.2 101.4 6.44 104.5 1.19 104.1 8.28 102.2 4.95 108.1 3.65 0.4 90.0 7.65 99.9 1.30 101.2 2.72 87.0 7.87 109.5 7.29 2 103.7 7.58 90.9 1.13 105.3 4.48 108.8 5.93 85.3 0.48 Alpha-zearalenol 0.2 106.2 5.34 98.2 6.43 110.3 1.74 82.8 7.91 109.9 1.25 0.4 91.5 6.54 105.7 7.03 83.4 5.43 81.0 7.65 109.2 4.64 2 79.7 0.79 104.2 4.02 104.0 0.18 110.5 1.66 87.4 1.61 Beta-zearalenol 0.2 106.8 5.92 93.5 2.68 82.6 1.32 100.6 4.57 107.8 2.84 0.4 98.5 6.81 107.2 5.50 79.0 3.42 95.5 2.51 105.4 6.64 2 80.3 7.27 104.0 3.89 80.7 1.92 80.5 5.42 88.4 5.08 Zearalanone 0.2 93.5 7.48 95.2 5.23 109.3 2.73 80.6 6.87 86.3 0.17 0.4 91.2 0.24 89.6 6.12 93.1 4.82 89.9 8.43 84.0 5.42 2 82.1 8.56 94.3 8.43 95.3 8.56 78.9 3.68 89.0 5.99 Zearalenone 0.2 93.2 2.53 109.9 2.94 105.8 6.35 73.7 3.58 90.2 8.86 0.4 86.1 0.82 87.0 0.12 97.6 4.99 82.6 5.11 100.1 0.13 2 72.8 6.24 100.8 2.90 70.2 4.26 104.7 3.23 103.9 1.12 Acetazolamide 1 95.5 8.72 97.3 2.81 102.3 6.94 89.4 1.14 91.5 9.00 2 91.4 4.88 98.5 8.01 103.2 1.06 98.7 7.43 106.3 1.66 10 107.8 3.36 97.8 3.59 99.0 3.76 99.3 8.50 92.1 6.99 Canrenone 1 110.2 5.07 90.0 4.73 110.0 6.32 102.8 0.81 100.8 5.82 2 102.9 6.08 107.9 8.77 108.8 4.10 88.3 6.79 109.6 7.06 10 103.1 6.03 106.2 2.54 88.6 8.89 101.0 1.25 97.8 3.09 Chlortalidone 1 106.4 7.40 109.6 1.36 103.6 5.33 91.5 7.71 103.1 3.40 2 102.4 1.87 86.8 7.06 110.0 4.49 90.5 5.74 92.0 5.11 10 98.5 0.30 93.7 0.05 105.4 5.78 93.0 8.66 109.7 4.09 Furosemide 1 110.3 7.63 101.0 1.30 91.9 7.21 109.4 1.70 98.9 8.98 2 105.2 2.19 96.7 0.94 94.2 0.31 100.8 6.27 98.4 2.03 10 97.4 8.51 98.2 1.96 98.8 8.26 90.5 7.19 103.8 2.38 Spironolactone 1 101.4 0.53 97.4 7.79 101.0 3.73 87.3 2.77 99.7 2.12 2 103.0 1.54 99.9 2.42 86.1 4.18 109.8 6.90 106.0 4.93 10 107.3 2.14 102.3 0.84 96.4 1.29 109.3 3.64 105.1 1.22 Bendroflumethiazide 1 102.9 7.71 102.9 0.20 101.8 3.28 97.0 5.11 110.7 3.25 2 106.4 3.01 101.2 4.28 93.9 1.34 105.2 6.64 105.6 8.23 10 102.2 5.28 101.2 6.59 108.2 2.48 99.0 0.83 86.7 6.24 Chlorothiazide 1 94.3 3.36 101.9 5.44 104.0 3.30 103.4 8.52 94.6 6.37 2 104.7 7.05 92.2 7.80 107.7 0.16 105.9 8.62 92.5 1.33 10 105.2 2.74 90.9 6.39 92.3 8.63 94.1 0.46 105.2 6.02 Hydrochlorothiazide 1 92.3 1.50 109.5 7.98 96.1 2.12 99.5 8.83 105.2 8.02 2 99.2 0.90 94.0 3.65 92.2 1.64 90.2 7.88 89.4 3.07 10 103.6 1.28 109.4 6.81 101.1 4.19 105.7 2.30 100.0 3.40 Triamterene 1 95.3 1.57 104.6 2.66 98.5 8.31 93.8 6.35 93.3 3.68 2 102.4 6.99 93.0 4.50 105.8 3.98 102.1 1.65 90.4 3.25 10 104.8 6.94 97.5 5.44 104.0 5.99 93.1 8.04 104.0 7.59 4-Amino-6-chlorobenzene-1,3-disulfonamide 1 95.4 1.41 110.7 8.06 106.9 2.00 107.1 5.87 107.0 2.38 2 95.4 5.29 101.9 4.42 89.0 0.70 103.3 0.57 91.7 2.31 10 103.5 0.73 108.8 2.07 94.2 4.31 110.6 6.07 92.3 4.79 Bumetanide 1 103.9 7.44 106.3 7.91 86.4 6.61 86.5 2.06 87.1 8.34 2 99.6 5.62 101.4 3.45 97.5 0.16 95.9 0.58 86.9 1.46 10 91.5 5.21 87.8 2.44 106.0 6.07 95.7 0.06 110.7 7.18 Torasemide 1 106.3 3.20 101.4 2.85 90.7 3.04 86.8 2.46 90.0 5.93 2 104.5 4.02 98.4 6.40 104.9 8.09 89.6 0.65 93.0 1.02 10 97.4 0.60 102.4 4.09 85.8 7.39 97.7 2.70 91.2 5.51 Acebutolol 0.2 107.4 5.30 108.5 0.50 106.7 3.39 108.9 7.65 110.7 6.45 0.4 100.4 2.89 101.5 3.33 104.1 4.94 99.8 0.70 107.6 8.56 2 95.0 0.84 93.7 2.33 108.2 3.35 93.4 8.09 94.4 5.25 Bromoclenbuterol 0.2 111.9 8.49 111.7 3.75 100.0 6.25 108.0 4.43 91.6 7.97 0.4 108.2 2.22 89.8 5.54 93.7 1.11 89.9 4.45 93.6 4.74 2 106.0 5.97 109.2 2.37 105.5 4.86 91.8 4.22 105.8 1.15 Celipolol 0.2 95.2 8.77 108.0 1.25 101.7 3.45 104.7 6.12 93.0 1.61 0.4 93.8 7.99 106.2 2.83 110.4 4.05 103.9 3.72 105.1 5.62 2 96.6 2.74 92.9 5.54 108.4 6.34 98.1 7.26 106.0 3.99 Formoterol 0.2 101.4 8.00 107.7 4.17 103.8 2.14 104.6 4.01 109.0 7.95 0.4 108.3 4.32 90.8 6.56 105.3 7.72 109.3 4.54 91.6 5.86 2 82.9 1.76 103.3 5.05 109.5 3.86 107.6 2.39 110.2 1.67 Brombuterol 0.2 110.8 4.91 104.3 2.33 110.8 6.07 108.2 3.53 103.5 3.37 0.4 101.1 7.70 109.4 6.29 102.5 3.03 105.7 0.47 89.0 0.32 2 110.7 4.67 98.9 7.59 99.3 8.10 93.4 5.30 103.9 2.87 Nebivolol 0.2 95.4 8.94 106.5 3.43 94.9 3.46 101.5 6.41 110.3 8.69 0.4 106.8 7.97 101.3 8.53 101.6 6.13 107.2 7.40 103.3 8.27 2 108.2 1.12 97.3 1.83 109.3 1.27 100.6 1.47 96.6 5.44
