Accurate Rapid Lifetime Determination on Time-Gated FLIM Microscopy with Optical Sectioning

Figure 5

Fluorescence lifetime images (a, b) and histograms (c, d) for HiLo processed images of capillary tubes filled with Erythrosine B (a, c) and Coumarin 153 (b, d) solutions, after running the IRF deconvolution algorithm. By comparing the histograms with the values presented in Table 1, it is possible to verify that the IRF deconvolution algorithm improves the accuracy of the fluorescence lifetimes measured with RLD.

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