[Retracted] Reversing Postcardiopulmonary Bypass Associated Cognitive Dysfunction Using k-Opioid Receptor Agonists to Regulate Microglial Polarization via the NLRP3/Caspase-1 Pathway

Figure 6

KORs suppress MI activation by regulating the NLRP3/caspase-1 pathway. (a) Western blotting was carried out to determine the protein expression levels of NLRP3 inflammasome and pro-caspase-1 in LPS-stimulated RM cells treated with caspase-1 inhibitor (AC-YVAD-cmk). LPS-stimulated RM cells were coadministered with the KOR agonist (U50488H) and excessively activated NLRP3 triggered by TLR4 agonist (RS09). The relative expression levels of NLRP3/caspase-1 pathway-related proteins (b) and MI-polarization-associated proteins (c) were quantified with western blotting. (d) Levels of inflammatory factors, M-CSF (pg/ml), IL-6 (pg/ml), and TNF-ɑ (pg/ml), in RM cell supernatant were determined using ELISA. All data in charts were represented as mean ± standard deviation; “ns” was labeled when the comparison between groups ( value) was greater than 0.05, while “” was labeled when the value was less than 0.05.

(a)

(b)

(c)

(d)