miR-130-3p Promotes MTX-Induced Immune Killing of Hepatocellular Carcinoma Cells by Targeting EPHB4

Figure 3

miR-130-3p overexpression promotes emission of DAMPs upon MTX treatment. The best segment shows the affiliation of safe cell invasion with EPHB4 expression. (b) Western smudge investigation of calreticulin after overexpression of miR-130-3p. (c) Immunodetection of CRT within the medium and extracellular calmodulin communicated by entirety cell lysis. (d) The energy of initiated ATP discharge. (e) Immunodetection of extracellular HMGB1 and HMGB1 in medium. (f) Time course of HMGB1 discharge after MTX treatment. Blunder bars imply ± s.d.

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