[Retracted] Helicid Reverses the Effect of Overexpressing NCALD, Which Blocks the sGC/cGMP/PKG Signaling Pathway in the CUMS-Induced Rat Model

Figure 6

The effect of AAV-siNCALD on the regulation of the sGC/cGMP/PKG signaling pathway and apoptosis cytokine caspase-3 using western blotting and an ELISA. (A1, B1, C1) Representative NCALD, sGCα1, sGCβ1, PKG1/2, cleaved-caspase-3, and β-actin bands. The relative protein levels of NCALD (A2), sGCα1 (C2), sGCβ1 (C3), PKG1/2 (C4), and cleaved-caspase-3 (B2), n = 3. ; the CUMS + SAL group vs. the CON + SAL group; ; , the CUMS + SAL group vs. the CUMS + AAV-siNCALD group. (D1) The level of cGMP in the hippocampus, n = 3. , the CUMS + SAL group vs. the CON + SAL group; , the CUMS + SAL group vs. the CUMS + AA V-siNCALD group. The results are expressed as the mean ± SEM.