[Retracted] Hyperleukocytic Acute Leukemia Circulating Exosomes Regulate HSCs and BM-MSCs

Figure 3

miR-125b inhibitor restrains the influence of HLAL-derived exosomes. (a) qPCR confirming miR-125b upregulation in HLAL-derived exosomes; compared with the NC group. (b), (c) After miR-125b inhibitor intervention, the miR-125b levels in HSCs and BM-MSCs treated with HLAL-exo were detected by RT-PCR; compared with the HSC/MSC + HLAL-exo group. (d) CFU assay indicated the effect of transfection with miR-125b inhibitor on the differentiation of HSCs when cocultured with HLAL-exo; compared with the HSC + HLAL-exo group. (e) Western blot assay showed the expression of a pattern of hematopoiesis-related genes in HSCs following transfection and HLAL-exo coculture; compared with the HSC group. and compared with the HSC + HLAL-exo group. (f) Western blot analysis performed on hematopoiesis-related genes following the suppression of miR-125b in BM-MSCs. compared with the MSC group. and , compared with the MSC + HLAL-exo group.

(a)

(b)

(c)

(d)

(e)

(f)