Tetramethylpyrazine Improves Monocrotaline-Induced Pulmonary Hypertension through the ROS/iNOS/PKG-1 Axis

<div>iNOS and PKG-1 expression in rat lungs. (a and b) iNOS and PKG-1 expression were measured by immunohistochemical analysis in three groups; there was a marked upregulation of iNOS (arrow in i-MCT) (a) and downregulation of PKG-1 (arrow in P-MCT) (b) in the lungs from MCT treated rats, downregulation iNOS and upregulation PKG-1after TMP treatment (arrow in i-MCT + TMP, P-MCT + TMP). (c) Western blot analysis demonstrated the iNOS and PKG-1 expression in rat lung homogenates. The bar graph showed iNOS and PKG-1 levels obtained from quantitative densitometry analysis. <i>N</i> = 12 per group. Data were mean ± SD, <svg height="15.3614pt" id="M5" style="vertical-align:-3.833899pt" version="1.1" viewbox="-0.0498162 -11.5275 54.2341 15.3614" width="54.2341pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.0091,0,0,-0.0091,0,-5.741)"><path d="M457 455H370L403 612H360L326 455H218L252 612H210L175 455H77L68 394H161L132 257H31L20 196H117L77 19H120L159 196H268L230 19H272L311 196H402L412 257H325L355 394H447L457 455ZM313 394L283 257H175L204 394H313Z"></path></g><g transform="matrix(.0091,0,0,-0.0091,4.432,-5.741)"><path d="M457 455H370L403 612H360L326 455H218L252 612H210L175 455H77L68 394H161L132 257H31L20 196H117L77 19H120L159 196H268L230 19H272L311 196H402L412 257H325L355 394H447L457 455ZM313 394L283 257H175L204 394H313Z"></path></g><g transform="matrix(.013,0,0,-0.013,9.395,.966)"><path d="M600 480C600 590 528 650 384 650H143L137 622C222 614 225 607 210 531L130 127C113 41 106 36 23 28L17 0H294L300 28C204 36 195 42 212 127L243 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602Z"></path></g><g transform="matrix(.013,0,0,-0.013,47.784,0)"><path d="M384 0V27C293 34 287 42 287 114V635C232 613 172 594 109 583V559L157 557C201 555 205 550 205 499V114C205 42 199 34 109 27V0H384Z"></path></g></svg> vs. control group. <svg height="10.7395pt" id="M6" style="vertical-align:-0.6370001pt" version="1.1" viewbox="-0.0498162 -10.1025 56.4993 10.7395" width="56.4993pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.0091,0,0,-0.0091,0,-5.741)"><path d="M486 158C486 177 478 202 466 220C413 228 386 236 336 262C386 288 413 297 466 304C478 323 486 347 485 366C470 376 444 381 422 380C389 338 368 319 321 288C323 345 329 372 349 422C339 442 322 461 305 470C289 461 271 442 262 422C281 372 287 345 290 288C243 319 222 338 189 380C167 381 142 376 125 366C125 347 133 322 145 304C198 296 225 288 275 262C225 236 198 227 145 220C133 201 125 177 126 158C141 148 167 143 189 144C222 186 243 205 290 236C288 179 282 152 262 102C272 82 289 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Journal of Healthcare Engineering

fig3

Figure 3

Figure 3: Tetramethylpyrazine Improves Monocrotaline-Induced Pulmonary Hypertension through the ROS/iNOS/PKG-1 Axis 