[Retracted] MicroRNA-489 Promotes the Apoptosis of Cardiac Muscle Cells in Myocardial Ischemia-Reperfusion Based on Smart Healthcare

Figure 5

miR-489 regulated cardiac muscle cells apoptosis after ischemia-reperfusion by targeting the SPIN1-mediated PI3K/AKT pathway. (a) The expression levels of AKT, p-AKT, and PIP3 in three groups of H9C2 cells were determined by Western blot to clarify the activation of the PI3K/A KT pathway. Increased expression levels of p-AKT and PIP3 in the miR-489 inhibitor group compared with those of the control and miR-489 NC groups suggest that miR-489 may play a biological role in inhibiting PI3K/AKT pathway. (b) H9C2 cells were transfected with PI3K/Akt pathway agonist SC79 to detect their apoptosis. Apoptosis decreased in the miR-489 inhibitor and SC79 groups compared with that of the control and miR-489 NC groups. (c) The expressions of apoptosis-related proteins in the SC79 and other groups were determined. Bcl-2 expression increased and the expressions of Bax, Cyt-C, and GSK3β decreased in SC79 and miR-489 inhibitor groups compared to the control and miR-489 NC groups.

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