Abnormal Function of Circulating Follicular Helper T Cells Leads to Different Manifestations of B Cell Maturation and Differentiation in Patients with Osteosarcoma

Figure 4

The effect of Tfh cells on B cell maturation and differentiation in Osteosarcoma patients. (a), ELISA detected the levels of IL-21, IL-10, and IL-4 in Tfh cells; (b), ELISA detected the levels of factors IgA, IgG, and IgM in B cells after co-culture; (c), qRT-PCR detected Blimp-1 levels in B cells after co-cultivation; (d), The levels of CD27 and CD38 in B cells after co-culture were identified by flow cytometry,  < 0.001 vs. HC group.

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